S220 Focused-ultrasonicator from Covaris LLC

Product Specs
ItemS220 Focused-ultrasonicator
CompanyCovaris LLC
Price Get Pricing Get Quote for this Product
Catalog Number500217
QuantityEA
Dimensions8” Width x 21” Depth x 13” Height (20 cm x 53 cm x 33 cm)
ApplicationsMechanical Shearing for Next Generation Sequencing (NGS) DNA/RNA Extraction from Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue Samples, Dried Blood Spots (DBS), and Whole Blood for NGS Chromatin Mechanical Shearing for ChIP-Seq Biomarker Extraction for Research and Clinical Microbiology Tissue disruption and homogenization Cell lysis Compound dissolution Particle micronization.
TypeFocused-ultrasonicator
Electrical Requirements100-240 VAC 500 VA, 50-60 Hz
Regulatory StatusCE, ETL Mark (for Product Safety), WEEE
TemperatureAmbient temperature: 19 to 25° (66 to 77° F) Relative humidity: 30% to 70%
Volume15 to 130 µl
ComponentsmicroTUBE:milliTUBE: miniTUBE:Large volume consumables:Flow cells

Add to Compare List

Covaris LLC

14 Gill St, Unit H

Woburn, Massachusetts 01801-1721

United States

Phone: +1 781-932-3959
Fax: +1 781-932-8705

Email: info@covaris.com

Company Profile

Website: www.covaris.com

(1) Comparison of global DNA methylation analysis by whole genome bisulfite sequencing and the Infinium Mouse Methylation BeadChip using fresh and fresh-frozen mouse epidermisEpigeneticsDecember 31, 2023Olivia Strunge Meyer, Mikkel Meyer Andersen, Claus Børsting, Niels Morling, Hans Christian Wulf, Peter Alshede Philipsen, Catharina Margrethe Lerche, Jeppe Dyrberg Andersenalso analysed with WGBS. For each sample, 100 ng genomic DNA was fragmented to 350 bp with the Covaris S220 Focused-ultrasonicator (Covaris). Subsequently, the DNA was bisulphite treated using the EZ DNA Methylation-Gold™ Kit (Zymo Research

(2) Case report: Genetic analysis of a novel intronic inversion variant in the SPTB gene associated with hereditary spherocytosisFront GenetDecember 4, 2023Bixin Xi, Siying Liu, Yongbing Zhu, Dedong Zhang, Yu Zhang, Aiguo LiuChina). Each genomic DNA sample was segmented into fragments with an average size of 150 bp using an S220 Focused-ultrasonicator (Covaris, Woburn, MA, United States). Then, the DNA Sample Prep Reagent Set (MyGenostics, Beijing, China)

(3) An optimized protocol for chromatin immunoprecipitation from murine inguinal white adipose tissueSTAR ProtocolsDecember 1, 2023Antonia Katsouda, Dimitrios Valakos, Giannis Vatsellas, Dimitris Thanos, Andreas PapapetropoulosmL,Kimble,057003 Cell strainer 200 μm,pluriSelect,43-50200-03 S220 Focused-ultrasonicator,Covaris,500217 microTUBE AFA Fiber Pre-Slit Snap-Cap 6 × 16 mm,Covaris,520045 16-Tube SureBeads Magnetic Rack,Bio-Rad,1614916 NovaSeq

(4) Delineating the interplay between oncogenic pathways and immunity in anaplastic Wilms tumorsNature CommunicationsNovember 30, 2023Xiaoping Su, Xiaofan Lu, Sehrish Khan Bazai, Linda Dainese, Arnauld Verschuur, Benoit Dumont, Roger Mouawad, Li Xu, Wenxuan Cheng, Fangrong Yan, et al.strand-displacement amplification. The resultant cDNA was fragmented to 200 bp (mean fragment size) with the S220 Focused-ultrasonicator (Covaris) and used to make barcoded sequencing libraries on the SPRI-TE Nucleic Acid Extractor (Beckman-Coulter).

(5) Accurate phenotypic classification and exome sequencing allow identification of novel genes and variants associated with adult-onset hearing lossPLOS GeneticsNovember 27, 2023Morag A. Lewis, Jennifer Schulte, Lois Matthews, Kenneth I. Vaden, Claire J. Steves, Frances M. K. Williams, Bradley A. Schulte, Judy R. Dubno, Karen P. SteelSureSelect Human All Exon V5 kit, which included 5’ and 3’ UTRs. DNA was sheared using the Covaris S220 focused ultrasonicator. Libraries were sequenced on the Illumina HiSeq 2500. The exome sequencing of the Twins UK cohort has

(6) Genetic profile of Chinese patients with small bowel cancer categorized by anatomic locationBMC Medical GenomicsNovember 16, 2023Chengmin Shi, Junrui Ma, Tong Zhang, Yanqiang Shi, Weiming Duan, Depei Huang, Hushan Zhang, Yujian Zengpreparation and targeted capture DNA extracts (30–200 ng) were sheared to 250 bp fragments using an S220 focused-ultrasonicator (Covaris). Libraries were prepared using the KAPA Hyper Prep Kit (KAPA Biosystems) following the manufacturer’s

(7) Irf7 regulates the expression of Srg3 and ferroptosis axis aggravated sepsis-induced acute lung injuryCellular & Molecular Biology LettersNovember 9, 2023Xinyu Ling, Shiyou Wei, Dandan Ling, Siqi Cao, Rui Chang, Qiuyun Wang, Zhize Yuanprotease inhibitors. This mixture was incubated at 4 °C for 30 min. To induce chromatin fragmentation, a COVARIS S220 focused-ultrasonicator was employed, resulting in DNA fragments spanning from 200 to 1000 base pairs. Subsequently, the chromatin

(8) Trained immunity of alveolar macrophages enhances injury resolution via KLF4-MERTK-mediated efferocytosisJ Exp MedNovember 6, 2023Sreeparna Chakraborty, Abhalaxmi Singh, Li Wang, Xinge Wang, Mark A. Sanborn, Zijing Ye, Mark Maienschein-Cline, Amitabha Mukhopadhyay, Balaji B. Ganesh, Asrar B. Malik, et al.formaldehyde followed by cell lysis. The nuclei portion was sonicated to fragment the DNA by using S220 Focused-ultrasonicator (Covaris). Further procedures were carried out using the Chromatin Immunoprecipitation (ChIP) Assay Kit

(9) Dried tea residue can alter the blood metabolism and the composition and functionality of the intestinal microbiota in Hu sheepFrontiers in MicrobiologyNovember 3, 2023Liangyong Guo, Shiqiang Yu, Fang Cao, Kaizhi Zheng, Manman Li, Zhenying Peng, Xingyun Shi, Liping LiuBiological Technology Co. Ltd. In briefly, for each sample, 1 μg of genomic DNA was sheared by Covaris S220 Focused-ultrasonicator (Woburn, MA USA) and sequencing libraries were prepared with a fragment length of approximately 450

(10) Defective EMC1 drives abnormal retinal angiogenesis via Wnt/β-catenin signaling and may be associated with the pathogenesis of familial exudative vitreoretinopathyGenes & DiseasesNovember 1, 2023Shujin Li, Mu Yang, Rulian Zhao, Li Peng, Wenjing Liu, Xiaoyan Jiang, Yunqi He, Erkuan Dai, Lin Zhang, Yeming Yang, et al.pathogenic genes for FEVR. Briefly, genomic DNA was fragmented to an average length of ∼150 bp using an S220 Focused-ultrasonicator (Covaris, USA). The standard Illumina libraries were prepared using an Agilent SureSelect Human All

(11) Euo is a developmental regulator that represses late genes and activates midcycle genes in Chlamydia trachomatismBioOctober 31, 2023Owais R. Hakiem, Syed M. A. Rizvi, Cuper Ramirez, Ming TanEDTA, 10 mM Tris-HCl (pH7.5), 0.1% SDS] without cross-linking. Cells were sonicated with a Covaris S220 Focused-ultrasonicator for 3 min to achieve an average fragment size of 150 bp. DNA fragments were purified by adding an equal

(12) Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh.International Journal of Molecular SciencesOctober 28, 2023Zhimin Lin, Muhammad Moaaz Ali, Xiaoyan Yi, Lijuan Zhang, Shaojuan Wang, Faxing ChenGermany) was used for DNA extraction. The genomic DNA was randomly interrupted at 200–300 bp using S220 Focused-ultrasonicator (Covaris, Brighton, UK); the interrupted DNA fragments were end-repaired, A-tailed, and ligated to sequencing

(13) Profound DNA methylomic differences between single- and multi-fraction alpha irradiations of lung fibroblastsClinical EpigeneticsOctober 27, 2023Marilyn N. Vera-Chang, John M. Danforth, Marilyne Stuart, Aaron A. Goodarzi, Marjorie Brand, Richard B. Richardsonbuffer, low EDTA, pH 8.0) was randomly sheared into fragments with an average size of 200 bp using an S220 focused-ultrasonicator (Covaris, USA). The sonication was performed at 7 °C for 180 s with a 10% duty factor, peak incident

(14) Predictive value of DNA methylation patterns in AML patients treated with an azacytidine containing induction regimenClinical EpigeneticsOctober 26, 2023Maximilian Schmutz, Manuela Zucknick, Richard F. Schlenk, Daniel Mertens, Axel Benner, Dieter Weichenhan, Oliver Mücke, Konstanze Döhner, Christoph Plass, Lars Bullinger, et al.[44]. In brief, a total of 3.0 μg DNA from bone marrow mononuclear cells was sonicated with the Covaris S220 focused-ultrasonicator (Covaris, Woburn, USA) to fragments of an optimal fragment size ranging between 100 and 200 bp as monitored

(15) Dynamic DNA methylation turnover in gene bodies is associated with enhanced gene expression plasticity in plantsGenome BiologyOctober 12, 2023Clara J. Williams, Dawei Dai, Kevin A. Tran, J. Grey Monroe, Ben P. WilliamsDNA samples with added spike-ins were sonicated to an average fragment size of 300 bp using a Covaris S220 Focused Ultrasonicator (80-s treatment, 140 peak power, 10 duty factor 200 cycles/burst). Subsequently, samples were incubated

(16) Unidirectional alteration of methylation and hydroxymethylation at the promoters and differential gene expression in oral squamous cell carcinomaFrontiers in GeneticsOctober 12, 2023Weizhi Zhao, Lin Zhu, Qian Gong, Suzhen Ma, Haofeng Xiong, Tong Su, Zhengqing Wan, Danling Wangμg genomic DNA mixed with unmethylated lambda DNA was fragmented to an average size of 250 bp with S220 Focused-ultrasonicator (Covaris, USA). The randomly fragmented DNA was subsequently subjected to constructed libraries using

(17) Museum DNA reveals a new, potentially extinct species of rinkhals (Serpentes: Elapidae: Hemachatus) from the Eastern Highlands of ZimbabwePLOS ONESeptember 27, 2023Tom Major, Pia Renk, Jens Reissig, Johanna L. A. Paijmans, Ellie Morris, Michael Hofreiter, Axel Barlow, Donald G. Broadley, Wolfgang Wüsterbaits were then prepared by pooling the PCR products in an equimolar ratio, shearing using a Covaris S220 focused ultrasonicator, after which biotinylated adapters were ligated following the procedures described in [30]. Using these

(18) Development of an in vitro genotoxicity assay to detect retroviral vector-induced lymphoid insertional mutantsMolecular Therapy - Methods & Clinical DevelopmentSeptember 14, 2023Antonella L. Bastone, Violetta Dziadek, Philipp John-Neek, Friederike Mansel, Jenni Fleischauer, Eric Agyeman-Duah, Dirk Schaudien, Oliver Dittrich-Breiholz, Adrian Schwarzer, Axel Schambach, et al.instructions. For INSPIIRED, between 70 and 1,000 ng of native or WGA amplified gDNA were sonicated using the S220 Focused-ultrasonicator (Covaris, Woburn, MA, USA), purified using AMPure beads in a 0.7-fold ratio, and end-repaired with the

(19) The minichromosome maintenance complex drives esophageal basal zone hyperplasiaJCI insightSeptember 8, 2023Rochman M, Rochman Y, Caldwell JM, Mack LE, Besse JA, Manes NP, Yoon SH, Shoda T, Nita-Lazar A, Rothenberg ME.were sonicated for 4 minutes in 110 μL of 8 M urea with protease inhibitors in a microTUBE using an S220 focused ultrasonicator (Covaris, LLC). Sonication conditions were used as recommended by the manufacturer (10% output, 70 W

(20) Chromatin remodeling of histone H3 variants by DDM1 underlies epigenetic inheritance of DNA methylationCellSeptember 1, 2023Seung Cho Lee, Dexter W. Adams, Jonathan J. Ipsaro, Jonathan Cahn, Jason Lynn, Hyun-Soo Kim, Benjamin Berube, Viktoria Major, Joseph P. Calarco, Chantal LeBlanc, et al.Extraction Kits (Cytiva). 1 μg gDNA samples were sheared to an average size of 400 bp using a Covaris S220 focused-ultrasonicator. BS-seq libraries were made using the NEXTFLEX Bisulfite Library Prep Kit (PerkinElmer). The library

Write a Review

(1)

11/17/2022Excellent Sonicator for Chromatin Sharing Filippo MassaOur studies focused on the epigenetic modification in different tissues (kidney, adrenal gland, renal precursors). Proper chromatin fragmentation is essential for the downstream analysis. This ... Read Review
(1)

3/23/2020Use of Covaris S220 Sonicator for Genomic Library Preparations Annie KesslerThe Covaris S220 sonicator is an efficient and user friendly device for the fragmentation of nucleic acid/genomic libraries for downstream sequencing applications.